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产品目录

14-3-3 Epsilon/YWHAE抗体图片
产品货号:
Z10825
中文名称:
14-3-3 Epsilon/YWHAE抗体
英文名称:
Anti-14-3-3 Epsilon/YWHAE Antibody
产品规格:
50μl|100μl|150μl
发货周期:
1~3天
产品价格:
询价
抗体名称Anti-14-3-3 Epsilon/YWHAE Antibody
指标别名14 3 3 protein epsilon;14 3 3E;14-3-3;14-3-3 epsilon;KCIP 1;MDCR;MDS;YWHAE
克隆性Polyclonal
检验物种human,mouse,rat
应用范围WB,IHC,FCM,ICC/IF,ELISA
基因名称YWHAE
抗体来源Rabbit
抗体类型IgG
免疫原E.coli-derived human YWHAE recombinant protein (Position:M1-Q255).
实际分子量29KD
成分500μg/mL antibody with PBS,0.02% NaN3,1mg BSA and 50% glycerol.
纯化方式Immunogen affinity purified.
浓度500μg/mL
产品形态溶液
保存条件12 months from date of receipt,-20℃ as supplied.6 months 2 to 8℃ after reconstitution.Avoid repeated freezing and thawing.
背景资料14-3-3 protein epsilon is a protein that in humans is encoded by the YWHAE gene.This gene product belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins.This highly conserved protein family is found in both plants and mammals,and this protein is 100% identical to the mouse ortholog.It interacts with CDC25 phosphatases,RAF1 and IRS1 proteins,suggesting its role in diverse biochemical activities related to signal transduction,such as cell division and regulation of insulin sensitivity.It has also been implicated in the pathogenesis of small cell lung cancer.Two transcript variants,one protein-coding and the other non-protein-coding,have been found for this gene.
Uniprot IDP62258
推荐配套试剂Biorab recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (ZN1926) for Western blot.
基因名全称tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein epsilon
蛋白名全称14-3-3 protein epsilon
推荐稀释比
Western blot (WB) 1:500~2000
Immunohistochemistry (IHC) 1:50~400
Immunocytochemistry/Immunofluorescence (ICC/IF) 1:50~400
Flow cytometry (FCM) 1~3μg/1×106 cells
Enzyme linked immunosorbent assay (ELISA) 1:100~1000
(Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user.

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